THE FUNCTIONAL STATE OF ACETYLCHOLINE-OPERATED IONIC CHANNELS DURING SEPARATE AND COMBINED EFFECT OF DECIS AND BELOPHOS ON HELIX POMATIA L. NEURONS

S.V. Vekovshinina

The study of the functional state of snail chemoexitable B6 neurone ionic channels using two microelectrodes and voltage clamp indicated that decis and belophos in concentrations 1 mM -1 mM caused a dose-dependent acetylcholine-induced inward current amplitude decrease. The above-mentioned chemicals do not change the linear character of the dependence between the evoked responses amplitude upon the voltage on the membrane and the ionic nature of the current being studied. The Hill coefficient estimation indicates the absence of cooperativity in decis and belophos binding to acetylcholine receptor complexes.
The combined effect of a pyrethroid and an organophosphorus compound in concentrations 0.1-1000 mM on the object being studied is characterized by potentiation of effects caused by each chemical separately. The combined effect evaluation using the dissociation constant-based Finney method suggests the incomplete summing.

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