THE PHARMACOLOGICAL CORRECTION OF SEPARATE COMPONENTS OF MI-TOCHONDRIAL AND MICROSOMAL ELECTRONTRANSPORT CHAINS LEVEL'S VIO-LATIONS IN HEPATOCYTES IN ACUTE TOXYCAL HEPATOPATHY CAUSED BY EPICHLORHYDRINE

I.Yu. Vysotsky

In this work the pharmacotherapeutic effectivity of medicines with different mechanisms of action in conditions of acute toxic hepatopathy caused by inhalational statical influence of epichlorhydrine (ECH) in concentration of 1/3 LC₅₀ is studied.
The article showes that the medical-prophylactic administration of quercetine (350 mg/kg) and flavinate (4 mg/kg) brought to increasing of iron-sulphuric proteins (ISP) level, also seven-quinone free radicals (FR), Mn²⁺- and Mo⁵⁺-containing paramagnetic complexes in liver tissue with simultaneous realistic decreasing of cytochrome P450 and nitrozyle com-plexes of iron (NCI) levels. Lipine (0,8 mmol/kg) and acetylcysteine (450 mg/kg) also in-creased the level of ISP but not influenced on FR level. Herewith, acetylcysteine at length in 12 and 72 hours after administration sharply decreased (in 7–29 times) the content of NCI and had variable influence upon level of cytochrome P450 and Mo⁵⁺-containing paramagnetic complexes. It is discovered that flavinate, less quercetine and acetylcysteine greatly restrained the declination of NADPH-cytochrom-c-reductase's activity in the microsomes of hepatocytes. The conclusion has been made that flavinate, quercetine and acetylcysteine can have positive influence upon oxydative phosphorylation in mitochondria and upon processes of detoxycation in microsomal system in the livers of animals, poisoned by ECH.

Keywords: epichlorhydrine, toxic hepatopathy, quercetine, flavinate, lipine, acetylcysteine, paramagnetic complexes, NADPH-cytochrome-c-reductase.

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